ULTRAMICRO METHOD AND SIMPLIFIED URINALYSIS

A rapid and specific ultramicro method for total serum cholesterol.

A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating ir.terference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic ac...

An ultramicro method for the estimation of plasma cholesterol.

A procedure is outlined for the ultramicro determination of total cholesterol that gives results in excellent agreement with classic methods. The determination requires 40 l. of plasma obtained from a finger-tip puncture and special handling of precipitated proteins. By the use of the described procedure plasma cholesterol levels have been estimated 4 times daily over a period of 8 weeks in you...

Ultramicro method for serum bilirubin by diazo blue reaction.

ROPER CLINICAL MANAGEMENT of infants with erythroblastosis fetalis necessitates the determination of total serum bilirubin. Of the many modifications of the original diazo reaction on blood serum (1) a micromethod using capillary blood is preferable. One tenth miffiliter of serum was used by Davidson et al. (2) in their modification of the Ernst and F#{246}rster (3) method and by With (4, 5) in...

Modified colorimetric ultramicro method for estimating Nefa in serum.

A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 mul samples; it is thus suitable for work on newborn babies and small laborato...

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.